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Cross-Sectioning Crickets with a Femtosecond Laser

A scanning-electron micrograph is shown of a cricket's body, focusing on the head, which has been sliced off just above the eyes.

Unlike most cutting lasers, femtosecond lasers don’t vaporize materials; rather, they produce such short, intense bursts of light that the affected region is ablated without having the chance to heat its surroundings. This makes them good at cutting away material without damaging the surroundings, something [Ben Krasnow] exploited to cut cross-sections of samples while still in a scanning-electron microscope.

In this case, the samples were crickets, and before imaging they had to be prepared. First, the bodies were soaked in glutaraldehyde to cross-link the proteins and stabilize the structure. Next, a series of solvent exchanges replaced the water in the bodies with a low-surface-tension solvent; this meant that during the next step, drying, surface tension wouldn’t distort the crickets’ internal structure. Finally, the insect bodies were charred under argon, which made the bodies conductive and more absorptive to laser light.

The laser itself and the scanning galvo are mounted outside the microscope, and shine in through a transparent window. To protect the detector and electron optics from a spray of ablated carbon, a servo motor swings an aluminium shutter between these and the sample while the laser is active. This caused some mysterious problems during testing: after the first ablation run, the electron microscope’s image would contain so much noise as to be unusable, but it would improve over time. As it turned out, the shutter was painted, and the other side of the paint was getting coated with charged carbon particles. This created a small capacitor which disrupted the electron optics as it discharged. Eventually, after solving this and a few other strange problems, [Ben] was able to take several time-lapse videos of the laser gradually ablating a cricket, 30 microns at a time, revealing its inner structure.

Although scanning-electron microscopes are unfortunately shard to come by, it’s still possible to restore a secondhand microscope or, as [Ben] did, build your own. Femtosecond lasers are yet more inaccessible, though they can be used to replicate themselves.

2026 Frikkin Lasers Challenge: Laser Bandsaw

Can you call it a bandsaw if it has neither band nor saw? [WeldingRod1] does, with his entry in the laser contest β€” a manually-controlled laser cutter that he’s dubbed a Laser Bandsaw. Some might quibble that it’s not actually sawing with the beam, and others will inevitably find the safety implications rather frightening. We think it’s a fun project and that [WeldingRod1] can call it what he wants, as long as he follows his own advice and keeps his laser goggles firmly on his precious vision orbs.

He has actually put some thought into what started as the physical manifestation of a joke in a podcast. The blue diode laser β€” a NUBM44 diode rated at 7 W β€” got a custom-made copper heatsink. It’s also got a hefty beam dump in the form of a stack of box knife blades. That’s very necessary to keep the beam from reflecting where it shouldn’t, especially when you consider this operates like a regular band saw: you turn it on, and it’s ready to cut. With only 7 W of laser power it can’t cut that much, mind you, but apparently it’s great on balsa wood and blasts black paint off like nobody’s business.

Now if this was our shop we’d probably want to put the laser diode onto some kind of CNC platform, be it Cartesian or SCARA. But we’ve seen that done many, many times and if you’ve got the motor skills this might be just the tool for you. There’s a pinout and STLs for the 3D printed frame on the project page if you’re interested. If not, why are you still here? The article is finished. Go make something lase and send it in. The deadline for the 2026 Frikkin Laser Contest is fast approaching!

2026 Hackaday Freaking Lasers Contest

Printing Fungal Art with Laser Control

A series of simulations of a shape are shown, with that shape traced out in a petri dish with a laser below. The shape is roughly like a 90-degree corner bisected by a third arm.

Preservationists usually take great care to prevent fungi from appearing the world of art, but in the case of [Kexin Wang]’s Funguy project, the fungus itself is the art. It uses a laser diode to repeatedly trace an outline onto a dish of agar gel in which fungus is growing, and the photophobic fungus grows only up to the edge of the laser-traced figure, potentially creating complex designs.

This project evolved out of a research project in which they developed a computer model for fungal growth, then used its predictions and a laser to control a fungus’s growth pattern. The model has two parts: a temporal convolutional neural network which learns fungi growth patterns from a series of images, and a cellular automaton to simulate these growth patterns under different starting conditions. The cellular automaton’s rules aren’t fixed; each cell runs a small neural network which learns the rules under supervision from the convolutional network. By training these networks on images of the growth stages of three different fungi, it was able to realistically predict the different growth patterns of the different species.

To actually control the growth pattern, the researchers tried a series of different wavelengths and laser powers; shorter wavelengths tended to work better, with a 405 nm laser working best. The growth model complemented the laser setup by predicting in which areas the growth medium had run out of nutrients. Since fungus would no longer spread in these regions, the laser no longer needed to trace these sections. The Funguy kit’s laser system itself is similar to a laser engraver, with an XY-kinematic system seemingly built from a DVD drive frame. It uses fungi from the Mucor genus, though it can print with other photophobic microorganisms, such as slime molds.

This project seems aimed at artistic and educational uses, but considering the various electronic parts that have been made of fungi, more functional applications should be possible.

2026 Frikkin Lasers Challenge: A 3D-Printed Raman Spectrometer

A black plastic cube is shown in front of another, larger rectangular black plastic box. The plastic cube has a silver microscope objective protruding from one side, with green light being emitted from it into a small plastic tube held on a positioning stage.

When light reflects off a surface, not all of it reflects off at the same wavelength; some photons impart a portion of their energy to raising the vibrational energy of the surface’s molecules, and are thus scattered away at a lower energy and longer wavelength. This is called Raman scattering, and the precise wavelength shifts are characteristic of the particular molecule being illuminated. It can therefore be used in Raman spectroscopy to identify molecules; these spectrometers are normally elaborate, expensive instruments, but [Allegedly Science] was able to build a simple system with surprising sensitivity.

The system is named the CubeRaman, after the cube-shaped body containing the main optical path. It uses a cheap 532-nm laser module as a monochromatic light source, with a bandpass filter to eliminate stray infrared light. The beam then reflects off a 45-degree dichroic mirror and passes through a microscope objective onto the sample. Raman-shifted light then scatters back through the objective, passes through the dichroic mirror and a long-pass optical filter, and is focused by an achromatic lens onto the slit of a spectrometer. The entire housing is 3D-printed, as are most parts of the kinematic mounts; the kinematic mounts use adjustment screws running through inserts in the mount, with the tips of the screws held in place by magnets.

[Allegedly Science]’s first test was with a raw diamond, which clearly showed the expected Raman shift. When trying to test a chemical inside a glass bottle, it mainly returned the signature of silica, making thin-walled cuvettes essential. Ethanol inside a plastic bottle was similarly interesting; varying the focal distance changed whether it detected the characteristic shift of ethanol or polypropylene. Nevertheless, [Allegedly Science] thinks there’s still room for improvement, particularly by eliminating stray light and using a narrower slit in the spectrometer.

Although we’ve seen an open-source Raman spectrometer before, this design is significantly more accessible. It does still require a separate spectrometer, though, so it might be worth considering some other spectrometer options.

2026 Hackaday Freaking Lasers Contest
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